ПЕРЕНОС ЧУЖЕРОДНЫХ ГЕНОВ СПЕРМАТОЗОИДАМИ В ЯЙЦЕКЛЕТКИ МИДИИ MYTILUS GALLOPROVINCIALIS LMK
Alien genes transfer by spermatozoids into ovicells of the mussel, Mytilus galloprovincialis Lmk
Alien genes transfer by spermatozoids into ovicells of the mussel, Mytilus galloprovincialis Lmk
Дата
1999
Название журнала
Номер ISSN
Название тома
Издатель
Севастополь: ЭКОСИ-Гидрофизика
Аннотация
Разработки в области генной инженерии морских беспозвоночных проводятся с учетом опыта, накопленного при конструировании генетически модифицированных микроорганизмов и при создании трансгенных растений и животных. В данной работе предпринята первая попытка генетической трансформации черноморской мидии Mytilus galloprovincialis Lmk.
Аннотация (alternative)
The possibility of exogenous DNA transfer by spermatozoids of the mussel Mytilus galloprovincialis Lmk. into ovicells was investigated in natural conditions and with the employment of several transfection methods. The rate of fertilization and larval growth were evaluated after DNA treatment of the spermatozoids. The study has shown a negative impact of the contact between mussel spermatozoids and exegenous DNA on the fertilization and the following development of embryos. During some experiments the number of developed larvae was reducing after DNA concentration and spermatozoid treatment term were increased. Group crossing permitted to document the facts of pCMVlacZ and pMTbGH plasmid transfer into the eggs. Alien DNA sequences were detected in the larvae 48 hours after the fertilization.
Ключевые слова
Библиографическое описание
Экология моря. - 1999. - Вып. 49 https://repository.marine-research.ru/handle/299011/4244